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All examples · Example 6 of 34

Strip PlotDistributionStatistical report

Strip Plot: qPCR ΔCt per Animal

ΔCt (target − GAPDH) for each animal in four treatment groups (n = 8 per group); lower ΔCt means higher target expression.

ΔCt (target − GAPDH) for each animal in four treatment groups (n = 8 per group); lower ΔCt means higher target expression.

Key findings

  • One-way ANOVA F = 67.11, p = 6.7 × 10⁻¹³, ω² = 0.861: dose explains most of the variation in ΔCt.
  • With n = 8 animals per group the minimum detectable effect is d = 1.51 at 80% power; every observed contrast is larger than that.
  • 6 of 6 pairwise contrasts survive Holm correction.
37 LabTools stats calls
11 of 15 stats methods
LabTools 0.14.3
600 dpi PNG Data (CSV) Raw LabTools output
In this reportDescriptive statisticsNormalityEqual variancesOutlier screenConfidence intervals for the meanDo the groups differ? (omnibus tests)Which groups differ? (pairwise, corrected)

Descriptive statistics

Location, spread and shape for every group — the first thing to read before choosing a test. Skewness near 0 and excess kurtosis near 0 are what a normal distribution looks like.

GroupnMeanSDSEMMedianQ1 – Q3Min – MaxSkewnessExcess kurtosis
Vehicle86.1250.48180.17036.2255.893 – 6.55.16 – 6.62-1.181.34
Low dose85.0810.4670.16515.0854.86 – 5.4184.3 – 5.67-0.412-0.493
Mid dose84.0260.29020.10264.033.947 – 4.1053.51 – 4.53-0.07051.75
High dose83.3180.430.1523.3053.11 – 3.6432.69 – 3.85-0.288-1.1
LabTools call
stats.describe
labtools stats describe --groups @groups.json

The same method is available as an MCP tool (stats_describe) and from the desktop app's statistics panel.

Normality

Three tests of the same question — could these values come from a normal distribution? Shapiro–Wilk has the best power at bench sample sizes, Anderson–Darling is more sensitive in the tails, and Kolmogorov–Smirnov (Lilliefors-corrected) is included for comparability with older papers. A significant p means normality is rejected.

GroupnShapiro–Wilk WpAnderson–Darling A²pK–S DpVerdict
Vehicle80.89840.280.36370.340.17040.71Consistent with normal
Low dose80.96060.820.18760.860.13120.95Consistent with normal
Mid dose80.94910.700.33830.400.19010.54Consistent with normal
High dose80.9310.530.25610.620.16030.79Consistent with normal
LabTools calls (3)
stats.normality
labtools stats normality --groups @groups.json --method shapiro-wilk
stats.normality
labtools stats normality --groups @groups.json --method anderson-darling
stats.normality
labtools stats normality --groups @groups.json --method kolmogorov-smirnov

The same method is available as an MCP tool (stats_normality) and from the desktop app's statistics panel.

LabTools notes

  • Group 'High dose' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.
  • Group 'Low dose' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.
  • Group 'Mid dose' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.
  • Group 'Vehicle' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.

Equal variances

Student's t and one-way ANOVA assume every group has the same spread. LabTools' Levene test uses deviations from the group median (the Brown–Forsythe form), which stays reliable on skewed data; Bartlett's test is more powerful but only trustworthy when the data are normal. A significant p means the equal-variance assumption fails — prefer Welch's t-test or a rank-based test.

TestStatisticpLargest / smallest varianceVerdict
Levene (Brown–Forsythe, median-centred)0.73570.542.76Homogeneous
Bartlett1.8690.602.76Homogeneous
LabTools calls (2)
stats.levene
labtools stats levene --groups @groups.json --method levene
stats.levene
labtools stats levene --groups @groups.json --method bartlett

The same method is available as an MCP tool (stats_levene) and from the desktop app's statistics panel.

LabTools notes

  • Group 'High dose' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.
  • Group 'Low dose' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.
  • Group 'Mid dose' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.
  • Group 'Vehicle' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.
  • Non-significant. This test has low power on small samples, so treat this as 'no evidence against equal variances', not as evidence for them.
  • Bartlett's test assumes normality and is badly inflated when that fails — confirm with stats.normality, or use method='levene'.

Outlier screen

Five detectors with different assumptions. Grubbs tests the single most extreme point, generalised ESD looks for up to several (immune to masking), Dixon's Q is the small-sample chemistry test, modified-z uses the median and MAD so needs no normality, and the IQR rule is Tukey's boxplot fence. LabTools flags points; it never deletes them.

MethodVehicleLow doseMid doseHigh dose
Grubbsnonenonenonenone
Generalised ESDnonenonenonenone
Dixon's Qnonenonenonenone
Modified z (MAD)nonenonenonenone
IQR fences (1.5×)nonenone2 (3.51, 4.53)none
LabTools calls (5)
stats.outliers
labtools stats outliers --groups @groups.json --method grubbs
stats.outliers
labtools stats outliers --groups @groups.json --method generalized-esd --max-outliers 5
stats.outliers
labtools stats outliers --groups @groups.json --method dixon-q
stats.outliers
labtools stats outliers --groups @groups.json --method modified-z
stats.outliers
labtools stats outliers --groups @groups.json --method iqr

The same method is available as an MCP tool (stats_outliers) and from the desktop app's statistics panel.

LabTools notes

  • Flagged points are candidates, not verdicts, and nothing has been removed. Look for a physical cause before excluding anything; if there is none, keep the point and use a robust test instead. Any exclusion must be reported.
  • The IQR rule is descriptive — it has no p-value and flags roughly 0.7% of a perfectly normal sample by construction.

Confidence intervals for the mean

t-based intervals: the range of population means these data are consistent with. Report them alongside p-values — an interval shows the size of an effect, a p-value does not.

GroupnMean95% CI
Vehicle86.125[5.722, 6.528]
Low dose85.081[4.691, 5.472]
Mid dose84.026[3.784, 4.269]
High dose83.318[2.958, 3.677]
LabTools calls (2)
stats.ci
labtools stats ci --data @Vehicle.json --conf-level 0.95
stats.ci
labtools stats ci --data @High_dose.json --conf-level 0.95

The same method is available as an MCP tool (stats_ci) and from the desktop app's statistics panel.

Do the groups differ? (omnibus tests)

One-way ANOVA compares means and assumes normal residuals with equal variances; Kruskal–Wallis compares ranks and assumes neither. Both only say the groups differ somewhere — the pairwise table below says where.

TestStatisticdfpEffect size
One-way ANOVAF = 67.113, 286.7 × 10⁻¹³ω² = 0.861 · η² = 0.878 · Cohen's f = 2.68
Kruskal–Wallis HH = 27.7834.0 × 10⁻⁶ε² = 0.896
LabTools calls (2)
stats.anova
labtools stats anova --groups @groups.json
stats.kruskal
labtools stats kruskal --groups @groups.json

The same method is available as an MCP tool (stats_anova) and from the desktop app's statistics panel.

LabTools notes

  • Group 'High dose' has n = 8; normality screening has very low power at this size, so a non-significant Shapiro–Wilk is weak evidence of normality.

Which groups differ? (pairwise, corrected)

6 comparisons (every pair), each run as a Welch t-test and a Mann–Whitney test. Testing many pairs inflates false positives, so every p-value is corrected with Holm (family-wise) and Benjamini–Hochberg (false-discovery rate) through stats.adjust.

ComparisonMean diff.95% CIHedges' gWelch pHolmBHMann–Whitney pHolmCliff's δ
Vehicle vs Low dose1.044[0.5349, 1.553]2.080.00060.0010.00070.0030.0060.906
Vehicle vs Mid dose2.099[1.663, 2.534]4.992.9 × 10⁻⁷1.5 × 10⁻⁶8.8 × 10⁻⁷0.00090.0061
Vehicle vs High dose2.807[2.317, 3.298]5.817.9 × 10⁻⁹4.7 × 10⁻⁸4.7 × 10⁻⁸0.00090.0061
Low dose vs Mid dose1.055[0.6303, 1.48]2.570.00020.00050.00030.0010.0060.969
Low dose vs High dose1.764[1.282, 2.245]3.711.8 × 10⁻⁶7.0 × 10⁻⁶3.5 × 10⁻⁶0.00090.0061
Mid dose vs High dose0.7088[0.3101, 1.107]1.830.0020.0020.0020.0030.0060.891
LabTools calls (3)
stats.ttest
labtools stats ttest --a @Vehicle.json --b @Low_dose.json --test welch --no-assumption-checks
stats.mannwhitney
labtools stats mannwhitney --a @Vehicle.json --b @Low_dose.json
stats.adjust
labtools stats adjust --p-values @p_values.json --labels @labels.json --method holm

The same method is available as an MCP tool (stats_ttest) and from the desktop app's statistics panel.

Design sensitivity

d = 1.51
Minimum detectable effect (80% power, α = 0.05)
n = 8 vs 8
15.4%
Power to detect d = 0.5
32.0%
Power to detect d = 0.8
60.8%
Power to detect d = 1.2
LabTools calls (2)
stats.power
labtools stats power --n1 8 --n2 8 --mode mde --power 0.8
stats.power
labtools stats power --n1 8 --n2 8 --effect-size 0.5

The same method is available as an MCP tool (stats_power) and from the desktop app's statistics panel.

Methods paragraph

Written to go straight into a manuscript.

Groups were compared by one-way ANOVA (F(3,28) = 67.1, p < 0.0001, ω² = 0.86); variances were homogeneous (Levene p = 0.54).

Every number on this page was computed by the LabTools statistics engine (version 0.14.3) from the example's CSV on 2026-09-23. Where a value was derived before the data reached LabTools (replicate means, paired differences, log odds ratios), the text says so. The data are simulated from a fixed random seed.

Swarm Plot: Calcium Transient AmplitudeRidgeline Plot: Cell Size over Time

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